RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ECE-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124274-100
Catalog Number: LS-G124274
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ECE1 (NM_001113349), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ECE-1 | ECE | ET-converting enzyme-1 | Endothelin converting enzyme 1 | Endothelin-converting enzyme | ET-1 converting enzyme-1 | ECE1 | Endothelin-converting enzyme 1 | HECE-1
- Origin Species
- Human
- NCBI GenBank
- NM_001397
- SwissProt
- P42892
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEALRESVLH LALQMSTYKR ATLDEEDLVD SLSEGDAYPN GLQVNFHSPR SGQRCWAART61QVEKRLVVLV VLLAAGLVAC LAALGIQYQT RSPSVCLSEA CVSVTSSILS SMDPTVDPCH121DFFSYACGGW IKANPVPDGH SRWGTFSNLW EHNQAIIKHL LENSTASVSE AERKAQVYYR181ACMNETRIEE LRAKPLMELI ERLGGWNITG PWAKDNFQDT LQVVTAHYRT SPFFSVYVSA241DSKNSNSNVI QVDQSGLGLP SRDYYLNKTE NEKVLTGYLN YMVQLGKLLG GGDEEAIRPQ301MQQILDFETA LANITIPQEK RRDEELIYHK VTAAELQTLA PAINWLPFLN TIFYPVEINE361SEPIVVYDKE YLEQISTLIN TTDRCLLNNY MIWNLVRKTS SFLDQRFQDA DEKFMEVMYG421TKKTCLPRWK FCVSDTENNL GFALGPMFVK ATFAEDSKSI ATEIILEIKK AFEESLSTLK481WMDEETRKSA KEKADAIYNM IGYPNFIMDP KELDKVFNDY TAVPDLYFEN AMRFFNFSWR541VTADQLRKAP NRDQWSMTPP MVNAYYSPTK NEIVFPAGIL QAPFYTRSSP KALNFGGIGV601VVGHELTHAF DDQGREYDKD GNLRPWWKNS SVEAFKRQTE CMVEQYSNYS VNGEPVNGRH661TLGENIADNG GLKAAYRAYQ NWVKKNGAEH SLPTLGLTNN QLFFLGFAQV WCSVRTPESS721HEGLITDPHS PSRFRVIGSL SNSKEFSEHF RCPPGSPMNP PHKCEVWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
767 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
