RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
EBF4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124203-100
Catalog Number: LS-G124203
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EBF4 (NM_001110514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EBF-4 | KIAA1442 | RP5-860F19.3 | Transcription factor COE4 | O/E-4 | Olf-1/EBF-like 4 | COE4 | Early B-cell factor 4 | EBF4 | OE-4
- Origin Species
- Human
- NCBI GenBank
- NM_001110514
- SwissProt
- Q9BQW3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDALPRSGLN LKEEPLLPAG LGSVRSWMQG AGILDASTAA QSGVGLARAH FEKQPPSNLR61KSNFFHFVLA MYDRQGQPVE VERTAFIDFV EKDREPGAEK TNNGIHYRLR LVYNNGLRTE121QDLYVRLIDS MSKQAIIYEG QDKNPEMCRV LLTHEIMCSR CCDRKSCGNR NETPSDPVII181DRFFLKFFLK CNQNCLKNAG NPRDMRRFQV VVSTTVSVDG HVLAVSDNMF VHNNSKHGRR241ARRLDPSEAA TPCIKAISPG EGWTTGGATV IVIGDNFFDG LQVVFGNVLV WSELITPHAI301RVQTPPRHIP GVVEVTLSYK SKQFCKGCPG RFVYTALNEP TIDYGFQRLQ KVIPRHPGDP361ERLPKEVLLK RAADLAEALY GVPGSNQELL LKRAADVAEA LYSTPRAPGP LAPLAPSHPH421PAVVGINAFS SPLAIAVGDA TPGPEPGYAR SCSSASPRGF APSPGSQQSG YGGGLGAGLG481GYGAPGVAGL GVPGSPSFLN GSTATSPFAI MPSSPPLAAA SSMSLPAAAP TTSVFSFSPV541NMISAVKQRS AFAPVLRPPS SPPQACPRAH GEGLPDQSFE DSDKFHSPAR GLQGLAYSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
598 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
