RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DYRK4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100146-100
Catalog Number: LS-G100146
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYRK4 (NM_003845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein kinase DYRK4 | DYRK4
- Origin Species
- Human
- NCBI GenBank
- AF263541
- SwissProt
- Q9NR20
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPASELKASE IPFHPSIKTQ DPKAEEKSPK KQKVTLTAAE ALKLFKNQLS PYEQSEILGY61AELWFLGLEA KKLDTAPEKF SKTSFDDEHG FYLKVLHDHI AYRYEVLETI GKGSFGQVAK121CLDHKNNELV ALKIIRNKKR FHQQALMELK ILEALRKKDK DNTYNVVHMK DFFYFRNHFC181ITFELLGINL YELMKNNNFQ GFSLSIVRRF TLSVLKCLQM LSVEKIIHCD LKPENIVLYQ241KGQASVKVID FGSSCYEHQK VYTYIQSRFY RSPEVILGHP YDVAIDMWSL GCITAELYTG301YPLFPGENEV EQLACIMEVL GLPPAGFIQT ASRRQTFFDS KGFPKNITNN RGKKRYPDSK361DLTMVLKTYD TSFLDFLRRC LVWEPSLRMT PDQALKHAWI HQSRNLKPQP RPQTLRKSNS421FFPSETRKDK VQGCHHSSRK ADEITKETTE KTKDSPTKHV QHSGDQQDCL QHGADTVQLP481QLVDAPKKSE AAVGAEVSMT SPGQSKNFSL KNTNVLPPIVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
