RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DOK3 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109726-100
Catalog Number: LS-G109726
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DOK3 (NM_024872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Docking protein 3 | DOK3 | Dok-like protein | DOKL
- Origin Species
- Human
- NCBI GenBank
- NM_024872
- SwissProt
- Q7L591
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRGARLRSD ARAQLNQLSL DGGTGSGQKG KCEEFPSSLS SVSPGLEAAA LLLAVTMDPL61ETPIKDGILY QQHVKFGKKC WRKVWALLYA GGPSGVARLE SWEVRDGGLG AAGDRSAGPG121RRGERRVIRL ADCVSVLPAD GESCPRDTGA FLLTTTERSH LLAAQHRQAW MGPICQLAFP181GTGEASSGST DAQSPKRGLV PMEENSIYSS WQEVGEFPVV VQRTEAATRC QLKGPALLVL241GPDAIQLREA KGTQALYSWP YHFLRKLGSD KGVFSFEAGR RCHSGEGLFA FSTPCAPDLC301RAVAGAIARQ RERLPELTRP QPCPLPRATS LPSLDTPGEL REMPPGPEPP TSRKMHLAEP361GPQSLPLLLG PEPNDLASGL YASVCKRASG PPGNEHLYEN LCVLEASPTL HGGEPEPHEG421PGSRSPTTSP IYHNGQDLSW PGPANDSTLE AQYRRLLELD QVEGTGRPDP QAGFKAKLVT481LLSRERRKGP APCDRPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
