RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNAJB5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125365-100
Catalog Number: LS-G125365
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNAJB5 (NM_001135004), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Heat shock protein cognate 40 | Hsc40 | Heat shock cognate 40 | Heat shock protein Hsp40-2 | Heat shock protein Hsp40-3 | DNAJB5
- Origin Species
- Human
- NCBI GenBank
- NM_012266
- SwissProt
- O75953
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.69 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGAEAEPWG RAPGPAIGGR RAGDSCPGWR RRSRSRGRGQ RLSHGPRRRP QLLTAAPPLQ61ARGAFRSFPH SWGEDFLASL MFKIQLEPLK LRAWTLNGFV KFRNKETSAG PVAVMGKDYY121KILGIPSGAN EDEIKKAYRK MALKYHPDKN KEPNAEEKFK EIAEAYDVLS DPKKRGLYDQ181YGEEGLKTGG GTSGGSSGSF HYTFHGDPHA TFASFFGGSN PFDIFFASSR STRPFSGFDP241DDMDVDEDED PFGAFGRFGF NGLSRGPRRA PEPLYPRRKV QDPPVVHELR VSLEEIYHGS301TKRMKITRRR LNPDGRTVRT EDKILHIVIK RGWKEGTKIT FPKEGDATPD NIPADIVFVL361KDKPHAHFRR DGTNVLYSAL ISLKEALCGC TVNIPTIDGR VIPLPCNDVI KPGTVKRLRG421EGLPFPKVPT QRGDLIVEFK VRFPDRLTPQ TRQILKQHLP CSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
462 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
