RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNA2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119324-100
Catalog Number: LS-G119324
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNA2 (NM_001080449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNA2-like helicase | DNA2L | DNA2 | HDNA2 | KIAA0083
- Origin Species
- Human
- NCBI GenBank
- D42046
- SwissProt
- P51530
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 129.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTPCIPSPK QGSKGRGQSP AAQVRGPTWN APAREGRPGA GHLGHLRVGA CARGAQARAF61FPFLSFLCYP RFRCLFCLQF AIPASRMEQL NELELLMEKS FWEEAELPAE LFQKKVVASF121PRTVLSTGMD NRYLVLAVNT VQNKEGNCEK RLVITASQSL ENKELCILRN DWCSVPVEPG181DIIHLEGDCT SDTWIIDKDF GYLILYPDML ISGTSIASSI RCMRRAVLSE TFRSSDPATR241QMLIGTVLHE VFQKAINNSF APEKLQELAF QTIQEIRHLK EMYRLNLSQD EIKQEVEDYL301PSFCKWAGDF MHKNTSTDFP QMQLSLPSDN SKDNSTCNIE VVKPMDIEES IWSPRFGLKG361KIDVTVGVKI HRGYKTKYKI MPLELKTGKE SNSIEHRSQV VLYTLLSQER RADPEAGLLL421YLKTGQMYPV PANHLDKREL LKLRNQMAFS LFHRISKSAT RQKTQLASLP QIIEEEKTCK481YCSQIGNCAL YSRAVEQQMD CSSVPIVMLP KIEEETQHLK QTHLEYFSLW CLMLTLESQS541KDNKKNHQNI WLMPASEMEK SGSCIGNLIR MEHVKIVCDG QYLHNFQCKH GAIPVTNLMA601GDRVIVSGEE RSLFALSRGY VKEINMTTVT CLLDRNLSVL PESTLFRLDQ EEKNCDIDTP661LGNLSKLMEN TFVSKKLRDL IIDFREPQFI SYLSSVLPHD AKDTVACILK GLNKPQRQAM721KKVLLSKDYT LIVGMPGTGK TTTICTLVRI LYACGFSVLL TSYTHSAVDN ILLKLAKFKI781GFLRLGQIQK VHPAIQQFTE QEICRSKSIK SLALLEELYN SQLIVATTCM GINHPIFSRK841IFDFCIVDEA SQISQPICLG PLFFSRRFVL VGDHQQLPPL VLNREARALG MSESLFKRLE901QNKSAVVQLT VQYRMNSKIM SLSNKLTYEG KLECGSDKVA NAVINLRHFK DVKLELEFYA961DYSDNPWLMG VFEPNNPVCF LNTDKVPAPE QVEKGGVSNV TEAKLIVFLT SIFVKAGCSP1021SDIGIIAPYR QQLKIINDLL ARSIGMVEVN TVDKYQGRDK SIVLVSFVRS NKDGTVGELL1081KDWRRLNVAI TRAKHKLILL GCVPSLNCYP PLEKLLNHLN SEKLIIDLPS REHESLCHIL1141GDFQRETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1146 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
