Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DLK1 / Pref-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100140-100
Catalog Number: LS-G100140
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DLK(DLK1) (NM_003836), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Delta-like protein dlk | DLK-1 | DLK1 | Delta1 | FA1 | Dlk | Preadipocyte factor 1 | Pref-1 | Protein delta homolog 1 | PG2 | ZOG | Fetal antigen 1 | PREF1 | Secredeltin
- Origin Species
- Human
- NCBI GenBank
- NM_003836
- SwissProt
- P80370
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.23 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTATEALLRV LLLLLAFGHS TYGAECFPAC NPQNGFCEDD NVCRCQPGWQ GPLCDQCVTS61PGCLHGLCGE PGQCICTDGW DGELCDRDVR ACSSAPCANN GTCVSLDDGL YECSCAPGYS121GKDCQKKDGP CVINGSPCQH GGTCVDDEGR ASHASCLCPP GFSGNFCEIV ANSCTPNPCE181NDGVCTDIGG DFRCRCPAGF IDKTCSRPVT NCASSPCQNG GTCLQHTQVS YECLCKPEFT241GLTCVKKRAL SPQQVTRLPN GYGLAYRLTP GVHELPVQQP EHRILKVSMK ELNKKTPLLT301EGQAICFTIL GVLTSLVVLG TVGIVFLNKC ETWVSNLRYN HMLRKKKNLL LQYNSGEDLA361VNIIFPEKID MTTFSKEAGD EEITRTRPLEQKLISEEDLAANDILDYKDDDDKV
383 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.