Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DDX4 / VASA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130032-100
Catalog Number: LS-G130032
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX4(DDX4) (NM_001166534), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX4 | DEAD box protein 4 | Vasa homolog | VASA
- Origin Species
- Human
- NCBI GenBank
- S75275
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSRNLFLTN SPESSNDCED NPTRNRGFSK RGDNDLDPDE CMQRTGGLFG SRRPVLSGTG61NGDTSQSRSG SGSERDSWKS EAEGGESSDT QGPKVTYIPP PPPEDEDSIF AHYQTGINFD121KYDTILVEVS GHDAPPAILT FEEANLCQTL NNNIAKAGYT KLTPVQKYSI PIILAGRDLM181ACAQTGSGKT AAFLLPILAH MMHDGITASR FKELQEPECI IVAPTRELVN QIYLEARKFS241FGTCVRAVVI YGGTQLGHSI RQIVQGCNIL CATPGRLMDI IGKEKIGLKQ IKYLVLDEAD301RMLDMGFGPE MKKLISCPGM PSKEQRQTLM FSATFPEEIQ RLAAEFLKSN YLFVAVGQVG361GACRDVQQTV LQVGQFSKRE KLVEILRNIG DERTMVFVET KKKADFIATF LCQEKISTTS421IHGDREQRER EQALGDFRFG KCPVLVATSV AARGLDIENV QHVINFDLPS TIDEYVHRIG481RTGRCGNTGR AISFFDLESD NHLAQPLVKV LTDAQQDVPA WLEEIAFSTY IPGFSGSTRG541NVFASVDTRK GKSTLNTAGF SSSQAPNPVD DESWDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
575 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.