Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCX / Doublecortin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123116-100
Catalog Number: LS-G123116
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Doublecortin(DCX) (NM_000555), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DBCN | Doublecortin | Doublin | Doublecortex | Lis-X | SCLH | LISX | XLIS | DCX | Lissencephalin-X
- Origin Species
- Human
- NCBI GenBank
- NM_000555
- SwissProt
- O43602
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTLPLHSHC TEMQRLLPKL EMLTLGSSFC SLQGEFCQAM DSFTTVSHVG MCEETDASFN61VFSPKFQFDR SHCQSLRFHQ NMELDFGHFD ERDKTSRNMR GSRMNGLPSP THSAHCSFYR121TRTLQALSNE KKAKKVRFYR NGDRYFKGIV YAVSSDRFRS FDALLADLTR SLSDNINLPQ181GVRYIYTIDG SRKIGSMDEL EEGESYVCSS DNFFKKVEYT KNVNPNWSVN VKTSANMKAP241QSLASSNSAQ ARENKDFVRP KLVTIIRSGV KPRKAVRVLL NKKTAHSFEQ VLTDITEAIK301LETGVVKKLY TLDGKQVTCL HDFFGDDDVF IACGPEKFRY AQDDFSLDEN ECRVMKGNPS361ATAGPKASPT PQKTSAKSPG PMRRSKSPAD SANGTSSSQL STPKSKQSPI STPTSPGSLR421KHKVDLYLPL SLDDSDSLGD SMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
442 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.