LSBio
CPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CPS1 antibodies →
$725.00each
Catalog Number: LS-G124405-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CPS1 (NM_001122633), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Carbamoyl-phosphate synthase | CPS1 | CPSase I | CPSASE1
Origin Species
Human
NCBI GenBank
NM_001875
SwissProt
P31327

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
165.65 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPQIIKMTRI LTAFKVVRTL KTGFGFTNVT AHQKWKFSRP GIRLLSVKAQ TAHIVLEDGT
61KMKGYSFGHP SSVAGEVVFN TGLGGYPEAI TDPAYKGQIL TMANPIIGNG GAPDTTALDE
121LGLSKYLESN GIKVSGLLVL DYSKDYNHWL ATKSLGQWLQ EEKVPAIYGV DTRMLTKIIR
181DKGTMLGKIE FEGQPVDFVD PNKQNLIAEV STKDVKVYGK GNPTKVVAVD CGIKNNVIRL
241LVKRGAEVHL VPWNHDFTKM EYDGILIAGG PGNPALAEPL IQNVRKILES DRKEPLFGIS
301TGNLITGLAA GAKTYKMSMA NRGQNQPVLN ITNKQAFITA QNHGYALDNT LPAGWKPLFV
361NVNDQTNEGI MHESKPFFAV QFHPEVTPGP IDTEYLFDSF FSLIKKGKAT TITSVLPKPA
421LVASRVEVSK VLILGSGGLS IGQAGEFDYS GSQAVKAMKE ENVKTVLMNP NIASVQTNEV
481GLKQADTVYF LPITPQFVTE VIKAEQPDGL ILGMGGQTAL NCGVELFKRG VLKEYGVKVL
541GTSVESIMAT EDRQLFSDKL NEINEKIAPS FAVESIEDAL KAADTIGYPV MIRSAYALGG
601LGSGICPNRE TLMDLSTKAF AMTNQILVEK SVTGWKEIEY EVVRDADDNC VTVCNMENVD
661AMGVHTGDSV VVAPAQTLSN AEFQMLRRTS INVVRHLGIV GECNIQFALH PTSMEYCIIE
721VNARLSRSSA LASKATGYPL AFIAAKIALG IPLPEIKNVV SGKTSACFEP SLDYMVTKIP
781RWDLDRFHGT SSRIGSSMKS VGEVMAIGRT FEESFQKALR MCHPSIEGFT PRLPMNKEWP
841SNLDLRKELS EPSSTRIYAI AKAIDDNMSL DEIEKLTYID KWFLYKMRDI LNMEKTLKGL
901NSESMTEETL KRAKEIGFSD KQISKCLGLT EAQTRELRLK KNIHPWVKQI DTLAAEYPSV
961TNYLYVTYNG QEHDVNFDDH GMMVLGCGPY HIGSSVEFDW CAVSSIRTLR QLGKKTVVVN
1021CNPETVSTDF DECDKLYFEE LSLERILDIY HQEACGGCII SVGGQIPNNL AVPLYKNGVK
1081IMGTSPLQID RAEDRSIFSA VLDELKVAQA PWKAVNTLNE ALEFAKSVDY PCLLRPSYVL
1141SGSAMNVVFS EDEMKKFLEE ATRVSQEHPV VLTKFVEGAR EVEMDAVGKD GRVISHAISE
1201HVEDAGVHSG DATLMLPTQT ISQGAIEKVK DATRKIAKAF AISGPFNVQF LVKGNDVLVI
1261ECNLRASRSF PFVSKTLGVD FIDVATKVMI GENVDEKHLP TLDHPIIPAD YVAIKAPMFS
1321WPRLRDADPI LRCEMASTGE VACFGEGIHT AFLKAMLSTG FKIPQKGILI GIQQSFRPRF
1381LGVAEQLHNE GFKLFATEAT SDWLNANNVP ATPVAWPSQE GQNPSLSSIR KLIRDGSIDL
1441VINLPNNNTK FVHDNYVIRR TAVDSGIPLL TNFQVTKLFA EAVQKSRKVD SKSLFHYRQY
1501SAGKAA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1506 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.