Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAST / Calpastatin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G131097-100
Catalog Number: LS-G131097
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpastatin(CAST) (NM_001190442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpastatin | BS-17 | Calpain inhibitor | Sperm BS-17 component | CAST
- Origin Species
- Human
- NCBI GenBank
- NM_001750
- SwissProt
- P20810
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH61TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI121SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP181EVSDPMSSTY IEELGKREVT IPPKYRELLA KPIGPDDAID ALSSDFTCGS PTAAGKKTEK241EESTEVLKAQ SAGTVRSAAP PQEKKRKVEK DTMSDQALEA LSASLGTRQA EPELDLRSIK301EVDEAKAKEE KLEKCGEDDE TIPSEYRLKP ATDKDGKPLL PEPEEKPKPR SESELIDELS361EDFDRSECKE KPSKPTEKTE ESKAAAPAPV SEAVCRTSMC SIQSAPPEPA TLKGTVPDDA421VEALADSLGK KEADPEDGKP VMDKVKEKAK EEDREKLGEK EETIPPDYRL EEVKDKDGKP481LLPKESKEQL PPMSEDFLLD ALSEDFSGPQ NASSLKFEDA KLAAAISEVV SQTPASTTQA541GAPPRDTSQS DKDLDDALDK LSDSLGQRQP DPDENKPMED KVKEKAKAEH RDKLGERDDT601IPPEYRHLLD DNGQDKPVKP PTKKSEDSKK PADDQDPIDA LSGDLDSCPS TTETSQNTAK661DKCKKAASSS KAPKNGGKAK DSAKTTEETS KPKDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
695 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.