Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CASP12 / Caspase 12 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130895-100
Catalog Number: LS-G130895
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Caspase 12(CASP12) (NM_001191016), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Caspase 12 pseudogene 1 | CASP12 | Caspase 12 | CASP-12 | Caspase 12 (gene/pseudogene) | Inactive caspase-12 | CASP12P1
- Origin Species
- Human
- NCBI GenBank
- AF486844
- SwissProt
- Q6UXS9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MADEKPSNGV LVHMVKLLIK TFLDGIFDDL MENNVLNTDE IHLIGKCLKF VVSNAENLVD61DITETAQTAG KIFREHLWNS KKQLSSDISS DGEREANMPG LNIRNKEFNY LHNRNGSELD121LLGMRDLLEN LGYSVVIKEN LTAQEMETAL RQFAAHPEHQ SSDSTFLVFM SHSILNGICG181TKHWDQEPDV LHDDTIFEIF NNRNCQSLKD KPKVIIMQAC RGNGAGIVWF TTDSGKAGAD241THGRLLQGNI CNDAVTKAHV EKDFIAFKSS TPHNVSWRHE TNGSVFISQI IYYFREYSWS301HHLEEIFQKV QHSFETPNIL TQLPTIERLS MTRYFYLFPG NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
341 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.