Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ASUN / C12orf11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111937-100
Catalog Number: LS-G111937
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C12orf11(ASUN) (NM_018164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASUN | Mat89Bb | NET48 | Sarcoma antigen NY-SAR-95 | SPATA30 | C12orf11 | Protein asunder homolog
- Origin Species
- Human
- NCBI GenBank
- NM_018164
- SwissProt
- Q9NVM9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIFSESHKT VFVVDHCPYM AESCRQHVEF DMLVKNRTQG IIPLAPISKS LWTCSVESSM61EYCRIMYDIF PFKKLVNFIV SDSGAHVLNS WTQEDQNLQE LMAALAAVGP PNPRADPECC121SILHGLVAAV ETLCKITEYQ HEARTLLMEN AERVGNRGRI ICITNAKSDS HVRMLEDCVQ181ETIHEHNKLA ANSDHLMQIQ KCELVLIHTY PVGEDSLVSD RSKKELSPVL TSEVHSVRAG241RHLATKLNIL VQQHFDLAST TITNIPMKEE QHANTSANYD VELLHHKDAH VDFLKSGDSH301LGGGSREGSF KETITLKWCT PRTNNIELHY CTGAYRISPV DVNSRPSSCL TNFLLNGRSV361LLEQPRKSGS KVISHMLSSH GGEIFLHVLS SSRSILEDPP SISEGCGGRV TDYRITDFGE421FMRENRLTPF LDPRYKIDGS LEVPLERAKD QLEKHTRYWP MIISQTTIFN MQAVVPLASV481IVKESLTEED VLNCQKTIYN LVDMERKNDP LPISTVGTRG KGPKRDEQYR IMWNELETLV541RAHINNSEKH QRVLECLMAC RSKPPEEEER KKRGRKREDK EDKSEKAVKD YEQEKSWQDS601ERLKGILERG KEELAEAEII KDSPDSPEPP NKKPLVEMDE TPQVEKSKGP VSLLSLWSNR661INTANSRKHQ EFAGRLNSVN NRAELYQHLK EENGMETTKN GKASRQTRTR PLEQKLISEE721DLAANDILDY KDDDDKV
737 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.