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AP1M2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AP1M2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115929-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AP1M2 (NM_005498), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AP-1 complex subunit mu-2 | AP-mu chain family member mu1B | AP1M2 | AP1-mu2 | HSMU1B | HA1 47 kDa subunit 2 | MU1B | Mu1B-adaptin | Mu2 | MU-1B | Mu-adaptin 2
Origin Species
Human
NCBI GenBank
NM_005498
SwissProt
Q9Y6Q5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MSASAVFILD VKGKPLISRN YKGDVAMSKI EHFMPLLVQR EEEGALAPLL SHGQVHFLWI
61KHSNLYLVAT TSKNANASLV YSFLYKTIEV FCEYFKELEE ESIRDNFVIV YELLDELMDF
121GFPQTTDSKI LQEYITQQSN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN
181LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGLSGSKN KSVELEDVKF
241HQCVRLSRFD NDRTISFIPP DGDFELMSYR LSTQVKPLIW IESVIEKFSH SRVEIMVKAK
301GQFKKQSVAN GVEISVPVPS DADSPRFKTS VGSAKYVPER NVVIWSIKSF PGGKEYLMRA
361HFGLPRVEKE EVEGRPPIGV KFEIPYFTVS GIQVRYMKII EKSGYQALPW VRYITQSGDY
421QLRTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

425 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.