Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANXA7 / Annexin VII / SNX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99348-100
Catalog Number: LS-G99348
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Annexin VII(ANXA7) (NM_001156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Annexin VII | Annexin-7 | SYNEXIN | Annexin A7 | ANX7 | ANXA7 | SNX
- NCBI GenBank
- NM_004034
- SwissProt
- P20073
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYPGYPPTG YPPFPGYPPA GQESSFPPSG QYPYPSGFPP MGGGAYPQVP SSGYPGAGGY61PAPGGYPAPG GYPGAPQPGG APSYPGVPPG QGFGVPPGGA GFSGYPQPPS QSYGGGPAQV121PLPGGFPGGQ MPSQYPGGQP TYPSQPATVT QVTQGTIRPA ANFDAIRDAE ILRKAMKGFG181TDEQAIVDVV ANRSNDQRQK IKAAFKTSYG KDLIKDLKSE LSGNMEELIL ALFMPPTYYD241AWSLRKAMQG AGTQERVLIE ILCTRTNQEI REIVRCYQSE FGRDLEKDIR SDTSGHFERL301LVSMCQGNRD ENQSINHQMA QEDAQRLYQA GEGRLGTDES CFNMILATRS FPQLRATMEA361YSRMANRDLL SSVSREFSGY VESGLKTILQ CALNRPAFFA ERLYYAMKGA GTDDSTLVRI421VVTRSEIDLV QIKQMFAQMY QKTLGTMIAG DTSGDYRRLL LAIVGQTRTR PLEQKLISEE481DLAANDILDY KDDDDKV
497 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.