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ANGPTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANGPTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANGPTL1 antibodies →
$504.00each
Catalog Number: LS-G100366-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANGPTL1(ANGPTL1) (NM_004673), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Angioarrestin | ANG-3 | Angiopoietin-like 1 | ANGPT3 | Angiopoietin-3 | Angiopoietin-like protein 1 | Angiopoietin-related protein 1 | ANGPTL1 | AngY | ARP1 | DJ595C2.2 (angiopoietin Y1) | KIAA0351 | ANG3 | Angiopoietin 3 | Angiopoietin Y1 | UNQ162 | DJ595C2.2
Origin Species
Human
NCBI GenBank
NM_004673
SwissProt
O95841

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.72 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKTFTWTLGV LFFLLVDTGH CRGGQFKIKK INQRRYPRAT DGKEEAKKCA YTFLVPEQRI
61TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID VLQLVVDVDG NIVNEVKLLR
121KESRNMNSRV TQLYMQLLHE IIRKRDNSLE LSQLENKILN VTTEMLKMAT RYRELEVKYA
181SLTDLVNNQS VMITLLEEQC LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ
241RDPGYPRDLM PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN
301SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW LGLENIYMLS
361NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG TYQGNAGDSM MWHNGKQFTT
421LDRDKDMYAG NCAHFHKGGW WYNACAHSNL NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL
481RAVQMMIKPI D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.