RBP4 Protein (Native)
Catalog Number: LS-G132074
2 sizes / from $793.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGAP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107494-100
Catalog Number: LS-G107494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGAP8(AGAP4) (NM_133446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGAP-4 | AGAP4 | AGAP-8 | AGAP8 | CTGLF5 | Em:AC012044.1 | MRIP2 | ARF GTPase-activating protein | CTGLF1
- Origin Species
- Human
- NCBI GenBank
- NM_133446
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNILTCRVH PSVSLEFDQQ QGSVCPSESE IYEAGAGDRM AGAPMAAAVQ PAEVTVEVGE61DLHMHHVRDR EMPEALEFNP SANPEASTIF QRNSQTDVVE IRRSNCTNHV STVRFSQQYS121LCSTIFLDDS TAIQHYLTMT IISVTLEIPH HITQRDADRS LSIPDEQLHS FAVSTVHIMK181KRNGGGSLNN YSSSIPSTPS TSQEDPQFSV PPTANTPTPV CKRSMRWSNL FTSEKGSDPD241KERKAPENHA DTIGSGRAIP IKQGMLLKRS GKWLKTWKKK YVTLCSNGVL TYYSSLGDYM301KNIHKKEIDL QTSTIKVPGK WPSLATSACT PISTSKSNGL SKDMDTGLGD SICFSPSISS361TTSPKLNPPP SPHANKKKHL KKKSTNNFMI VSATGQTWHF EATTYEERDA WVQAIQSQIL421ASLQSCESSK SKSQLTSQSK AMALQSIQNM RGNAHCVDCE TQNPKWASLN LGVLMCIECS481GIHRSLGTRL SRVRSLELDD WPVELRKVMS SIGNDLANSI WEGSSQGQTK PSEKSTREEK541ERWIRSKYEE KLFLAPLPCT ELSLGQQLLR ATADEDLQTA ILLLAHGSRE EVNETCGEGD601GCTALHLACR KGNVVLAQLL IWYGVDVMAR DAHGNTALTY ARQASSQECI NVLLQYGCPD661KCVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
663 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
