Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACSL4 / FACL4 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100301-100
Catalog Number: LS-G100301
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FACL4(ACSL4) (NM_004458), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Acyl-CoA synthetase 4 | ACS4 | ACSL4 | FACL4 | LACS4 | LACS 4 | Lignoceroyl-CoA synthase | MRX63 | MRX68
- Origin Species
- Human
- NCBI GenBank
- NM_004458
- SwissProt
- O60488
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKRIKAKPT SDKPGSPYRS VTHFDSLAVI DIPGADTLDK LFDHAVSKFG KKDSLGTREI61LSEENEMQPN GKVFKKLILG NYKWMNYLEV NRRVNNFGSG LTALGLKPKN TIAIFCETRA121EWMIAAQTCF KYNFPLVTLY ATLGKEAVVH GLNESEASYL ITSVELLESK LKTALLDISC181VKHIIYVDNK AINKAEYPEG FEIHSMQSVE ELGSNPENLG IPPSRPTPSD MAIVMYTSGS241TGRPKGVMMH HSNLIAGMTG QCERIPGLGP KDTYIGYLPL AHVLELTAEI SCFTYGCRIG301YSSPLTLSDQ SSKIKKGSKG DCTVLKPTLM AAVPEIMDRI YKNVMSKVQE MNYIQKTLFK361IGYDYKLEQI KKGYDAPLCN LLLFKKVKAL LGGNVRMMLS GGAPLSPQTH RFMNVCFCCP421IGQGYGLTES CGAGTVTEVT DYTTGRVGAP LICCEIKLKD WQEGGYTIND KPNPRGEIVI481GGQNISMGYF KNEEKTAEDY SVDENGQRWF CTGDIGEFHP DGCLQIIDRK KDLVKLQAGE541YVSLGKVEAA LKNCPLIDNI CAFAKSDQSY VISFVVPNQK RLTLLAQQKG VEGTWVDICN601NPAMEAEILK EIREAANAMK LERFEIPIKV RLSPEPWTPE TGLVTDAFKL KRKELRNHYL661KDIERMYGGKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
670 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.