Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ACOX1 / ACOX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130096-100
Catalog Number: LS-G130096
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACOX1 (NM_001185039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACOX | ACOX1 | Acyl-CoA oxidase 1 | palmitoyl | Acyl-coa oxidase | peroxisomal | AOX | SCOX | PALMCOX | Palmitoyl-CoA oxidase
- Origin Species
- Human
- NCBI GenBank
- NM_004035
- SwissProt
- Q15067
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MILNDPDFQH EDLNFLTRSQ RYEVAVRKSA IMVKKMREFG IADPDEIMWF KNFVHRGRPE61PLDLHLGMFL PTLLHQATAE QQERFFMPAW NLEIIGTYAQ TEMGHGTHLR GLETTATYDP121ETQEFILNSP TVTSIKWWPG GLGKTSNHAI VLAQLITKGK CYGLHAFIVP IREIGTHKPL181PGITVGDIGP KFGYDEIDNG YLKMDNHRIP RENMLMKYAQ VKPDGTYVKP LSNKLTYGTM241VFVRSFLVGE AARALSKACT IAIRYSAVRH QSEIKPGEPE PQILDFQTQQ YKLFPLLATA301YAFQFVGAYM KETYHRINEG IGQGDLSELP ELHALTAGLK AFTSWTANTG IEACRMACGG361HGYSHCSGLP NIYVNFTPSC TFEGENTVMM LQTARFLMKS YDQVHSGKLV CGMVSYLNDL421PSQRIQPQQV AVWPTMVDIN SPESLTEAYK LRAARLVEIA AKNLQKEVIH RKSKEVAWNL481TSVDLVRASE AHCHYVVVKL FSEKLLKIQD KAIQAVLRSL CLLYSLYGIS QNAGDFLQGS541IMTEPQITQV NQRVKELLTL IRSDAVALVD AFDFQDVTLG SVLGRYDGNV YENLFEWAKN601SPLNKAEVHE SYKHLKSLQS KLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
622 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.