Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ABHD16B / C20orf135 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107866-100
Catalog Number: LS-G107866
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABHD16B (NM_080622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_080622
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MCVICFVKAL VRVFKIYLTA SYTYPFRGWP VAFRWDDVRA VGRSSSHRAL TCAAAAAGVW61LLRDETLGGD ALGRPPRGAR SQAQCLLQQL RELPGQLASY ALAHSLGRWL VYPGSVSLMT121RALLPLLQQG QERLVERYHG RRAKLVACDG NEIDTMFMDR RQHPGSHVHG PRLVICCEGN181AGFYEMGCLS APLEAGYSVL GWNHPGFGSS TGVPFPQHDA NAMDVVVEYA LHRLHFPPAH241LVVYGWSVGG FTATWATMTY PELGALVLDA TFDDLVPLAL KVMPHSWKGL VVRTVREHFN301LNVAEQLCCY PGPVLLLRRT QDDVVSTSGR LRPLSPGDVE GNRGNELLLR LLEHRYPVVM361AREGRAVVTR WLRAGSLAQE AAFYARYRVD EDWCLALLRS YRARCEEELE GEEALGPHGP421AFPWLVGQGL SSRRRRRLAL FLARKHLKNV EATHFSPLEP EEFQLPWRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
469 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.