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ABCB8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ABCB8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114804-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ABCB8 (NM_007188), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MABC1 | M-ABC1 | Mitochondrial ABC protein | EST328128
Origin Species
Human
NCBI GenBank
NM_007188
SwissProt
Q9NUT2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLVHLFRVGI RGGPFPGRLL PPLRFQTFSA VRYSDGYRSS SLLRAVAHLR SQLWAHLPRA
61PLAPRWSPSA WCWVGGALLG PMVLSKHPHL CLVALCEAEE APPASSTPHV VGSRFNWKLF
121WQFLHPHLLV LGVAVVLALG AALVNVQIPL LLGQLVKVVA KYTRDHVGSF MTESQNLSTH
181LLILYGVQGL LTFGYLVLLS HVGERMAVDM RRALFSSLLR QNITFFDANK TGQLVSRLTT
241DVQEFKSSFK LVISQGLRSC SQVAGCLVSL SMLSTRLTLL LMVATPALMG VGTLMGSGLR
301KLSRQCQEHI ARAMGVADEA LGNVRTVRAL AMEQREEERY GAELEACRCR AEELGRGIAL
361FQGLSNIAFN CMVLGTLFIG GSLVAGQQLT GGDLMSFLVA SQTVQRSMAN LSVLFGQVVR
421GLSAGARVFE YMALNPCIPL SGGCCVPKEQ LRGSVTFQNV CFSYPCRPGF EVLKDFTLTL
481PPGKIVALVG QSGGGKTTVA SLLERFYDPT AGVVMLDGRD LRTLDPSWLR GQVVGFISQE
541PVLFGTTIME NIRFGKLEAS DEEVYTAARE ANAHEFITSF PEGYNTVVGE RGTTLSGGQK
601QRLAIARALI KQPTVLILDE ATSALDAESE RVVQEALDRA SAGRTVLVIA HRLSTVRGAH
661CIVVMADGRV WEAGTHEELL KKGGLYAELI RRQALDAPRT AAPPPKKPEG PRSHQHKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

718 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.