LSBio
ZXDA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZXDA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZXDA antibodies →
$504.00each
Catalog Number: LS-G114825-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZXDA (NM_007156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF896 | Zinc finger protein 896 | ZXDA
Origin Species
Human
NCBI GenBank
BC002940

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEIPKLLPAR GTLQGGGGGG IPAGGGRVHR GPDSPAGQVP TRRLLLPRGP QDGGPGRRRE
61EASTASRGPG PSLFAPRPHQ PSGGGDDFFL VLLDPVGGDV ETAGSGQAAG PVLREEAKAG
121PGLQGDESGA NPAGCSAQGP HCLSAVPTPA PISAPGPAAA FAGTVTIHNQ DLLLRFENGV
181LTLATPPPHA WEPGAAPAQQ PRCLIAPQAG FPQAAHPGDC PELRSDLLLA EPAEPAPAPA
241PQEEAEGLAA ALGPRGLLGS GPGVVLYLCP EALCGQTFAK KHQLKMHLLT HSSSQGQRPF
301KCPLGGCGWT FTTSYKLKRH LQSHDKLRPF GCPAEGCGKS FTTVYNLKAH MKGHEQENSF
361KCEVCEESFP TQAKLGAHQR SHFEPERPYQ CAFSGCKKTF ITVSALFSHN RAHFREQELF
421SCSFPGCSKQ YDKACRLKIH LRSHTGERPF LCDFDGCGWN FTSMSKLLRH KRKHDDDRRF
481MCPVEGCGKS FTRAEHLKGH SITHLGTKPF VCPVAGCCAR FSARSSLYIH SKKHLQDVDT
541WKSRCPISSC NKLFTSKHSM KTHMVKRHKV GQDLLAQLEA ANSLTPSSEL TSQRQNDLSD
601AEIVSLFSDV PDSTSAALLD TALVNSGILT IDVASVSSTL AGHLPANNNN SVGQAVDPPS
661LMATSDPPQS LDTSLFFGTA ATGFQQSSLN MDEVSSVSVG PLGSLDSLAM KNSSPEPQAL
721TPSSKLTVDT DTLTPSSTLC ENSVSELLTP AKAEWSVHPN SDFFGQEGET QFGFPNAAGN
781HGSQKERNLI TVTGSSFLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

799 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.