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ZW10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZW10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116455-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZW10 (NM_004724), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HZW10 | KNTC1AP | Zeste white 10 homolog | ZW10 | Zw10 kinetochore protein
Origin Species
Human
NCBI GenBank
NM_004724
SwissProt
O43264

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PSMQSAQGLI
61TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE RDSVVLSLLK QLQEFSTAIE
121EYNCALTEKK YVTGAQRLEE AQKCLKLLKS RKCFDLKILK SLSMELTIQK QNILYHLGEE
181WQKLIVWKFP PSKDTSSLES YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK
241LKSFGQMLLK YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL
301EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS IPTNSSKLQQ
361YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH
421NTVKIIPDSK INVPELPTPD EDNKLEVQKV SNTQYHEVMN LEPENTLDQH SFSLPTCRIS
481ESVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI
541HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL
601LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC KAMGTLLNTA
661ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SEESKNKKYQ EEVPVYVPKW
721MPFKELMMML QASLQEIGDR WADGKGPLAA AFSSSEVKAL IRALFQNTER RAAALAKIK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

779 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.