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ZUFSP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZUFSP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102306-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZUFSP (NM_145062), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf113 | RP3-412I7.3 | ZUFSP | DJ412I7.3
Origin Species
Human
SwissProt
Q96AP4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLSCNICGET VTSEPDMKAH LIVHMESEII CPFCKLSGVN YDEMCFHIET AHFEQNTLER
61NFERINTVQY GTSDNKKDNT LQCGMEVNSS ILSGCASNHP KNSAQNLTKD STLKHEGFYS
121ENLTESRKFL KSREKQSSLT EIKGSVYETT YSPPECPFCG KIEEHSEDME THVKTKHANL
181LDIPLEDCDQ PLYDCPMCGL ICTNYHILQE HVDLHLEENS FQQGMDRVQC SGDLQLAHQL
241QQEEDRKRRS EESRQEIEEF QKLQRQYGLD NSGGYKQQQL RNMEIEVNRG RMPPSEFHRR
301KADMMESLAL GFDDGKTKTS GIIEALHRYY QNAATDVRRV WLSSVVDHFH SSLGDKGWGC
361GYRNFQMLLS SLLQNDAYND CLKGMLIPCI PKIQSMIEDA WKEGFDPQGA SQLNNRLQGT
421KAWIGACEVY ILLTSLRVKC HIVDFHKSTG PLGTHPRLFE WILNYYSSEG EGSPKVVCTS
481KPPIYLQHQG HSRTVIGIEE KKNRTLCLLI LDPGCPSREM QKLLKQDIEA SSLKQLRKSM
541GNLKHKQYQI LAVEGALSLE EKLARRQASQ VFTAEKIP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.