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ZSWIM5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSWIM5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZSWIM5 antibodies →
$725.00each
Catalog Number: LS-G110930-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSWIM5 (NM_020883), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1511 | ZSWIM5
Origin Species
Human
NCBI GenBank
AA676667

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADGGEREEL LSPSPVSPAK RQCSWPSPQA HHPRGSPGAA GGGAGGVGSS CLVLGARPHL
61QPDSLLDCAA KTVAEKWAYE RVEERFERIP EPVQRRIVYW SFPRNEREIC MYSSFQYRGG
121PGAGAAGGAA GASPAEEGPQ PPPGAAAPAG SAPGGVAAGA SPGLGAGAGA AGCGGEGLPF
181RRGIRLLDSG SVENVLQVGF HLSGTVTELA TASEPAVTYK VAISFDRCKI TSVTCGCGNK
241DIFYCAHVVA LSLYRIRKPD QVKLRLPISE TLFQMNRDQL QKFIQYLITA HHTEVLPTAQ
301KLADEILSSN SEINQVNGAP DPTAGASIDD ENCWHLDEEQ VKEQVKLFLS QGGYCGSGKQ
361LNSMFAKVRE MLRMRDSNGA RMLTLITEQF VADPRLTLWR QQGTNMTDKC RQLWDELGAL
421WVCIILNPHC KLEEKSCWLQ QLQKWSDLDV CPLEDGNYGH ELPNITNALP QSAIHSPDSL
481SRPRRTVFTR AIEGRELHWQ DSHLQRIISS DVYTAPACQR ESERLLFNSQ GQPLWLEHVP
541TACARVDALR SHGYPKEALR LTVAIINTLR LQQQRQLEIY KHQKKELLQR GTTTITNLEG
601WVGHPLDPID CLFLTLTEAC RLNDDGYLEM SDMNESRPPV YQHVPVAAGS PNSSESYLSL
661ALEVALMGLG QQRLMPEGLY AQDKVCRNEE QLLSQLQELQ LDDELVQTLQ KQCILLLEGG
721PFSGLGEVIH RESVPMHTFA KYLFSALLPH DPDLSYKLAL RAMRLPVLEN SASAGDTSHP
781HHMVSVVPSR YPRWFTLGHL ESQQCELAST MLTAAKGDTL RLRTILEAIQ KHIHSSSLIF
841KLAQDAFKIA TPTDSSTDST LLNVALELGL QVMRMTLSTL NWRRREMVRW LVTCATEVGV
901RALVSILQSW YTLFTPTEAT SIVAATAVSH TTILRLSLDY PQREELASCA RTLALQCAMK
961DPQSCALSAL TLCEKDHIAF EAAYQIAIDA AAGGMTHSQL FTIARYMELR GYPLRAFKLA
1021SLAMSHLNLA YNQDTHPAIN DVLWACALSH SLGKNELAAL IPLVVKSVHC ATVLSDILRR
1081CTVTAPGLAG IPGRRSSGKL MSTDKAPLRQ LLDATINAYI NTTHSRLTHI SPRHYGEFIE
1141FLSKARETFL LPQDGHLQFA QFIDNLKQIY KGKKKLMLLV RERFG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1185 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.