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ZSWIM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSWIM4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZSWIM4 antibodies →
$725.00each
Catalog Number: LS-G110190-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSWIM4 (NM_023072), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZSWIM4
Origin Species
Human
NCBI GenBank
AK024452
SwissProt
Q9H7M6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPPAAKRSR GCPAGPEERD AGAGAARGRG RPEALLDLSA KRVAESWAFE QVEERFSRVP
61EPVQKRIVFW SFPRSEREIC MYSSLGYPPP EGEHDARVPF TRGLHLLQSG AVDRVLQVGF
121HLSGNIREPG SPGEPERLYH VSISFDRCKI TSVSCGCDNR DLFYCAHVVA LSLYRIRHAH
181QVELRLPISE TLSQMNRDQL QKFVQYLISA HHTEVLPTAQ RLADEILLLG SEINLVNGAP
241DPTAGAGIED ANCWHLDEEQ IQEQVKQLLS NGGYYGASQQ LRSMFSKVRE MLRMRDSNGA
301RMLILMTEQF LQDTRLALWR QQGAGMTDKC RQLWDELGAL WVCVVLSPHC KPEERAGWLQ
361LLSRWDKLDV CPLEEGNYSF DGPSLQPTMA PAPELLQKGS TCITNTEGWV GHPLDPIGCL
421CRALLEACRL EEETLTLYPD SGPEKRKVAY QHVPVPGSPG ESYLVLALEV ALLGLGQQRA
481LPEGLYAQDK VVRNEEQLLA LLEEVELDER LVQVLRKQAG LLLEGGPFSG FGEVLFRESV
541PMHTCARYLF TALLPHDPDL AYRLALRAMR LPILETAFPA GEPHPSPLDS IMSNRFPRWF
601ILGHLETRQC ELASTMLTAA KGDPKWLHTV LGSIQQNIHS PALLFKLAQD ACKTATPVSA
661PPDTTLLGIA LELGLQVMRM TLNVMTWRRR EMVRWLVSCA TEIGPQALMN IMQNWYSLFT
721PVEAATIVAV TGTTHATLLR LQLDTSRREE LWACARTLAL QCAMKDPQNC ALPALTLCEK
781NHSAFEAAYQ IVLDAAAGGL GHAHLFTVAR YMEHRGLPLR AYKLATLALA QLSIAFNQDS
841HPAVNDVLWA CSLSHSLGRH ELSAIVPLII RSIHCAPMLS DILRRWTLSA PGLGPLGARR
901AAKPLGADRA PLCQLLDAAV TAYITTSHSR LTHISPRHYG DFIEFLGKAR ETFLLAPDGH
961LQFSQFLENL KQTYKGKKKL MLLVRERFG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

989 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.