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ZSWIM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSWIM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107774-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSWIM3 (NM_080752), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf164 | DJ337O18.7 | ZSWIM3
Origin Species
Human
SwissProt
Q96MP5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELGSCFKTY EDFKECFSAY KRENRCSFIL RDCVSVRFHN LNHGTSIRED ILYVQVKFVC
61IRTQSNRKRT READMCPAYL LLRYNERLDR LFISELNTQH IHGDSKVASP GGDTTGKSQK
121TMCLQRLQPV QPTTKKDLDT AEKSLVEPSF CLDKVQVSSK PEQEGITPSD LAKIAKVMKN
181FLKVDEGSMA SFSVGDSQHL DRLSFQSSKM TDLFIRFPEN LLLHRVENTQ GHILYAFLVE
241NKERESRVVH FAVLKAETVT SVAKMLSIFT EFNSDWPKVK VVFVDPSFHY RAILQEIFPA
301ARILLSIYHT TRLLEKKLHR SSANPSFKRL MKEALREAVF VTSEASLKNL CQMSQAVLDE
361DLFNFLQAHW FTCELLWYMH VRKGLLACNT YMDSLDIVTS KVSSLFREQQ SLLDCILCFV
421DYIDFFNTKG LKNLPTPPPK LKRARPASMP LKSKKAFGIC GESLTSLPAE ETKPDAQQVQ
481VQQQSQVPPS QVGMLDTLHQ SGSELAYKLC HNEWEVVQNS THLVDMAGSS VDVQLLEDSH
541QVSKDGCSCS CSFQQWYHLP CRHILALLHT SQQPVGEAMV CRRWQKKYQY LLGPNGELQD
601RGMVPNTGQP EKQGRNDMIQ DLSRELANLL MQTEGPELEE RYSTLRKIVD IWAGPSQPSE
661LFQQPGDFKD VGRLPFLWGK QEEGEGFPPA TAVMHY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

696 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.