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ZSWIM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSWIM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107853-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSWIM1 (NM_080603), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf162 | DJ337O18.5 | ZSWIM1
Origin Species
Human
NCBI GenBank
NM_080603
SwissProt
Q9BR11

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLERLKAPWS AALQRKYFDL GIWTAPISPM ALTMLNGLLI KDSSPPMLLH QVNKTAQLDT
61FNYQSCFMQS VFDHFPEILF IHRTYNPRGK VLYTFLVDGP RVQLEGHLAR AVYFAIPAKE
121DTEGLAQMFQ VFKKFNPAWE RVCTILVDPH FLPLPILAME FPTAEVLLSA FHICKFLQAK
181FYQLSLERPV ERLLLTSLQS TMCSATAGNL RKLYTLLSNC IPPAKLPELH SHWLLNDRIW
241LAHRWRSRAE SSHYFQSLEV TTHILSQFFG TTPSEKQGMA SLFRYMQQNS ADKANFNQGL
301CAQNNHAPSD TIPESPKLEQ LVESHIQHSL NAICTGPAAQ LCLGELAVVQ KSTHLIGSGS
361EKMNIQILED THKVQPQPPA SCSCYFNQAF HLPCRHILAM LSARRQVLQP DMLPAQWTAG
421CATSLDSILG SKWSETLDKH LAVTHLTEEV GQLLQHCTKE EFERRYSTLR ELADSWIGPY
481EQVQLTRTRP LEQKLISEED LAANDILDYK DDDDKV

516 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.