LSBio
ZSCAN5B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSCAN5B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZSCAN5B antibodies →
$504.00each
Catalog Number: LS-G129006-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZSCAN5B (NM_001080456), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF495B | ZNF371 | ZSCAN5B
Origin Species
Human
NCBI GenBank
NM_001080456
SwissProt
A6NJL1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MAANWTLSWG QGGPCNSPGS DTPRSVASPE TQLGNHDRNP ETWHMNFRMF SCPEESDPIQ
61ALRKLTELCH LWLRPDLHTK EQILDMLVME QFMISMPQEL QVLVKVNGVQ SCKDLEDLLR
121NNRRPKKWSI VNLLGKEYLM LNSDVEMAEA PASVRDDPRD VSSQWASSVN QMHPGTGQAR
181REQQILPRVA ALSRRQGEDF LLHKSIDVTG DPNSPRPKQT LEKDLKENRE ENPGLSSPEP
241QLPKSPNLVR AKEGKEPQKR ASVENVDADT PSACVVEREA LTHSGNRGDA LNLSSPKRSK
301PDASSISQEE PQGEATPVGN RESPGQAEIN PVHSPGPAGP VSHPDGQEAK ALPPFACDVC
361NKSFKYFSQL SIHRRSHTGD RPFQCDLCRK RFLQPSDLRV HQRVHTGERP YMCDVCQKRF
421AHESTLQGHK RIHTGERPFK CKYCSKVFSH KGNLNVHQRT HSGEKPYKCP TCQKAFRQLG
481TFKRHLKTHR ETTSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.