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ZSCAN22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSCAN22 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZSCAN22 antibodies →
$504.00each
Catalog Number: LS-G104312-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF50(ZSCAN22) (NM_181846), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GLI-Kruppel family member HKR2 | ZNF50 | ZSCAN22 | Protein HKR2 | Zinc finger protein 50 | HKR2 | Kruppel related
Origin Species
Human
NCBI GenBank
M20676
SwissProt
P10073

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAIPKHSLSP VPWEEDSFLQ VKVEEEEEAS LSQGGESSHD HIAHSEAARL RFRHFRYEEA
61SGPHEALAHL RALCCQWLQP EAHSKEQILE LLVLEQFLGA LPPEIQAWVG AQSPKSGEEA
121AVLVEDLTQV LDKRGWDPGA EPTEASCKQS DLGESEPSNV TETLMGGVSL GPAFVKACEP
181EGSSERSGLS GEIWTKSVTQ QIHFKKTSGP YKDVPTDQRG RESGASRNSS SAWPNLTSQE
241KPPSEDKFDL VDAYGTEPPY TYSGKRSSKC RECRKMFQSA SALEAHQKTH SRKTPYACSE
301CGKAFSRSTH LAQHQVVHTG AKPHECKECG KAFSRVTHLT QHQRIHTGEK PYKCGECGKT
361FSRSTHLTQH QRVHTGERPY ECDACGKAFS QSTHLTQHQR IHTGEKPYKC DACGRAFSDC
421SALIRHLRIH SGEKPYQCKV CPKAFAQSSS LIEHQRIHTG EKPYKCSDCG KAFSRSSALM
481VHLRIHITVL Q
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.