Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZSCAN21 / Zipro1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106524-100
Catalog Number: LS-G106524
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP38(ZSCAN21) (NM_145914), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KOX25 | ZNF38 | NY-REN-21 | ZSCAN21 | ZFP38 | Zfp-38 | Zinc finger protein 38 homolog | Zipro1 | Zinc finger protein 38
- Origin Species
- Human
- NCBI GenBank
- NM_145914
- SwissProt
- Q9Y5A6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMTKVLGMAP VLGPRPPQEQ VGPLMVKVEE KEEKGKYLPS LEMFRQRFRQ FGYHDTPGPR61EALSQLRVLC CEWLRPEIHT KEQILELLVL EQFLTILPQE LQAWVQEHCP ESAEEAVTLL121EDLERELDEP GHQVSTPPNE QKPVWEKISS SGTAKESPSS MQPQPLETSH KYESWGPLYI181QESGEEQEFA QDPRKVRDCR LSTQHEESAD EQKGSEAEGL KGDIISVIIA NKPEASLERQ241CVNLENEKGT KPPLQEAGSK KGRESVPTKP TPGERRYICA ECGKAFSNSS NLTKHRRTHT301GEKPYVCTKC GKAFSHSSNL TLHYRTHLVD RPYDCKCGKA FGQSSDLLKH QRMHTEEAPY361QCKDCGKAFS GKGSLIRHYR IHTGEKPYQC NECGKSFSQH AGLSSHQRLH TGEKPYKCKE421CGKAFNHSSN FNKHHRIHTG EKPYWCHHCG KTFCSKSNLS KHQRVHTGEG EAPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
473 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.