Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZSCAN18 / ZNF447 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126759-100
Catalog Number: LS-G126759
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF447(ZSCAN18) (NM_001145542), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZSCAN18 | Zinc finger protein 447 | ZNF447
- Origin Species
- Human
- NCBI GenBank
- NM_023926
- SwissProt
- Q8TBC5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRVGGRADE DATAAGSLWV LAPPEPTGHL VIPGTSPLEP PCPWLDSHIF QCRFGKMLPL61EKAFASPRSS PAPPDLPTPG SAAGVQQEEP ETIPERTPAD LEFSRLRFRE FVYQEAAGPH121QTLARLHELC RQWLMPEARS KEQMLELLVL EQFLGILPDK VRPWVVAQYP ESCKKAASLV181EGLADVLEEP GMLLGSPAGS SSILSDGVYE RHMDPLLLPG ELASPSQALG AGEIPAPSET241PWLSPDPLFL EQRRVREAKT EEDGPANTEQ KLKSFPEDPQ HLGEWGHLDP AEENLKSYRK301LLLWGYQLSQ PDAASRLDTE ELRLVERDPQ GSSLPEGGRR QESAGCACEE AAPAGVLPEL361PTEAPPGDAL ADPPSGTTEE EEEQPGKAPD PQDPQDAESD SATGSQRQSV IQQPAPDRGT421AKLGTKRPHP EDGDGQSLEG VSSSGDSAGL EAGQGPGADE PGLSRGKPYA CGECGEAFAW481LSHLMEHHSS HGGRKRYACQ GCWKTFHFSL ALAEHQKTHE KEKSYALGGA RGPQPSTREA541QAGARAGGPP ESVEGEAPPA PPEAQRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
566 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.