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ZSCAN12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZSCAN12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120643-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF96(ZSCAN12) (NM_001039643), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ29K1.2 | KIAA0426 | ZNF29K1 | Zinc finger protein 305 | ZNF305 | ZNF96 | ZFP96 | Zinc finger protein 96 | ZSCAN12
Origin Species
Human
NCBI GenBank
AB007886
SwissProt
O43309

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASTWAIQAH MDQDEPLEVK IEEEKYTTRQ DWDLRKNNTH SREVFRQYFR QFCYQETSGP
61REALSRLREL CHQWLRPETH TKEQILELLV LEQFLTILPE ELQAWVQEQH PESGEEVVTV
121LEDLERELDE PGEQVSVHTG EQEMFLQETV RLRKEGEPSM SLQSMKAQPK YESPELESQQ
181EQVLDVETGN EYGNLKQEVS EEMEPHGKTS SKFENDMSKS ARCGETREPE EITEEPSACS
241REDKQPTCDE NGVSLTENSD HTEHQRICPG EESYGCDDCG KAFSQHSHLI EHQRIHTGDR
301PYKCEECGKA FRGRTVLIRH KIIHTGEKPY KCNECGKAFG RWSALNQHQR LHTGEKHYHC
361NDCGKAFSQK AGLFHHIKIH TRDKPYQCTQ CNKSFSRRSI LTQHQGVHTG AKPYECNECG
421KAFVYNSSLV SHQEIHHKEK CYQCKECGKS FSQSGLIQHQ RIHTGEKPYK CDVCEKAFIQ
481RTSLTEHQRI HTGERPYKCD KCGKAFTQRS VLTEHQRIHT GERPYKCDEC GNAFRGITSL
541IQHQRIHTGE KPYQCDECGK AFRQRKKTSY KEILLKNHSE PQAGVNLLLS SLIPEWQSCF
601RRDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

604 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.