Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZRANB2 / ZNF265 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115958-100
Catalog Number: LS-G115958
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZRANB2 (NM_005455), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Zinc finger | splicing | Zinc finger protein 265 | ZIS | ZNF265 | ZRANB2 | ZIS2 | Zinc-finger protein 265 | Zinc-finger | ZIS1
- Origin Species
- Human
- NCBI GenBank
- NM_005455
- SwissProt
- O95218
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTKNFRVSD GDWICPDKKC GNVNFARRTS CNRCGREKTT EAKMMKAGGT EIGKTLAEKS61RGLFSANDWQ CKTCSNVNWA RRSECNMCNT PKYAKLEERT GYGGGFNERE NVEYIEREES121DGEYDEFGRK KKKYRGKAVG PASILKEVED KESEGEEEDE DEDLSKYKLD EDEDEDDADL181SKYNLDASEE EDSNKKKSNR RSRSKSGSSH SRSSSRSSSP SSSRSRSRSR SRSSSSSQSR241SRSSSRERSR SRGSKSRSSS RSHRGSSSPR KRSYSSSSSS PERNRKRSRS RSSSSGDRKK301RRTRSRSPES QVIGENTKQPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
320 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.