Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZPBP / SP38 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114923-100
Catalog Number: LS-G114923
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZPBP1(ZPBP) (NM_007009), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZPBP1 | SP38 | Zona pellucida binding protein | ZPBP
- Origin Species
- Human
- NCBI GenBank
- NM_007009
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAFALGPAR RGRRRTRAAG SLLSRAAILL FISAFLVRVP SSVGHLVRLP RAFRLTKDSV61KIVGSTSFPV KAYVMLHQKS PHVLCVTQQL RNAELIDPSF QWYGPKGKVV SVENRTAQIT121STGSLVFQNF EESMSGIYTC FLEYKPTVEE IVKRLQLKYA IYAYREPHYY YQFTARYHAA181PCNSIYNISF EKKLLQILSK LLLDLSCEIS LLKSECHRVK MQRAGLQNEL FFAFSVSSLD241TEKGPKRCTD HNCEPYKRLF KAKNLIERFF NQQVEILGRR AEQLPQIYYI EGTLQMVWIN301RCFPGYGMNV QQHPKCPECC VICSPGSYNP RDGIHCLQCN SSLVYGAKTC LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
351 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.