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ZP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117318-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZP2 (NM_003460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zona pellucida glycoprotein 2 | ZP2 | ZPA | Zona pellucida protein A | Zp-2
Origin Species
Human
NCBI GenBank
NM_003460
SwissProt
Q05996

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACRQRGGSW SPSGWFNAGW STYRSISLFF ALVTSVNSID VSQLVNPAFP GTVTCDEREI
61TVEFPSSPGT KKWHASVVDP LGLDMPNCTY ILDPEKLTLR ATYDNCTRRV HGGHQMTIRV
121MNNSAALRHG AVMYQFFCPA MQVEETQGLS ASTICQKDFM SFSLPRVFSG LADDSKGTKV
181QMGWSIEVGD GARAKTLTLP EAMKEGFSLL IDNHRMTFHV PFNATGVTHY VQGNSHLYMV
241SLKLTFISPG QKVIFSSQAI CAPDPVTCNA THMTLTIPEF PGKLKSVSFE NQNIDVSQLH
301DNGIDLEATN GMKLHFSKTL LKTKLSEKCL LHQFYLASLK LTFLLRPETV SMVIYPECLC
361ESPVSIVTGE LCTQDGFMDV EVYSYQTQPA LDLGTLRVGN SSCQPVFEAQ SQGLVRFHIP
421LNGCGTRYKF EDDKVVYENE IHALWTDFPP SKISRDSEFR MTVKCSYSRN DMLLNINVES
481LTPPVASVKL GPFTLILQSY PDNSYQQPYG ENEYPLVRFL RQPIYMEVRV LNRDDPNIKL
541VLDDCWATST MDPDSFPQWN VVVDGCAYDL DNYQTTFHPV GSSVTHPDHY QRFDMKAFAF
601VSEAHVLSSL VYFHCSALIC NRLSPDSPLC SVTCPVSSRH RRATGATEAE KMTVSLPGPI
661LLLSDDSSFR GVGSSDLKAS GSSGEKSRSE TGEEVGSRGA MDTKGHKTAG DVGSKAVAAV
721AAFAGVVATL GFIYYLYEKR TVSNH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

745 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.