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ZNRF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNRF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108994-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNRF3 (NM_032173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BK747E2.3 | RING finger protein 203 | RNF203 | ZNRF3 | Zinc/RING finger protein 3 | KIAA1133 | Zinc and ring finger 3
Origin Species
Human
NCBI GenBank
AK025710

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHPLGLCNNN DEEDLYEYGW VGVVKLEQPE LDPKPCLTVL GKAKRAVQRG ATAVIFDVSE
61NPEAIDQLNQ GSEDPLKRPV VYVKGADAIK LMNIVNKQKV ARARIQHRPP RQPTEYFDMG
121IFLAFFVVVS LVCLILLVKI KLKQRRSQNS MNRLAVQALE KMETRKFNSK SKGRREGSCG
181ALDTLSSSST SDCAICLEKY IDGEELRVIP CTHRFHRKCV DPWLLQHHTC PHCRHNIIEQ
241KGNPSAVCVE TSNLSRGRQQ RVTLPVHYPG RVHRTNAIPA YPTRTSMDSH GNPVTLLTMD
301RHGEQSLYSP QTPAYIRSYP PLHLDHSLAA HRCGLEHRAY SPAHPFRRPK LSGRSFSKAA
361CFSQYETMYQ HYYFQGLSYP EQEGQSPPSL APRGPARAFP PSGSGSLLFP TVVHVAPPSH
421LESGSTSSFS CYHGHRSVCS GYLADCPGSD SSSSSSSGQC HCSSSDSVVD CTEVSNQGVY
481GSCSTFRSSL SSDYDPFIYR SRSPCRASEA GGSGSSGRGP ALCFEGSPPP EELPAVHSHG
541AGRGEPWPGP ASPSGDQVST CSLEMNYSSN SSLEHRGPNS STSEVGLEAS PGAAPDLRRT
601WKGGHELPSC ACCCEPQPSP AGPSAGAAGS STLFLGPHLY EGSGPAGGEP QSGSSQGLYG
661LHPDHLPRTD GVKYEGLPCC FYEEKQVARG GGGGSGCYTE DYSVSVQYTL TEEPPPGCYP
721GARDLSQRIP IIPEDVDCDL GLPSDCQGTH SLGSWGGTRG PDTPRPHRGL GATREEERAL
781CCQARALLRP GCPPEEAGAV RANFPSALQD TQESSTTATE AAGPRSHSAD SSSPGA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

836 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.