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ZNF862 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF862 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF862 antibodies →
$725.00each
Catalog Number: LS-G119010-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF862 (NM_001099220), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0543 | ZNF862 | Zinc finger protein 862
Origin Species
Human
NCBI GenBank
AB011115
SwissProt
O60290

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPRESGKAP VTFDDITVYL LQEEWVLLSQ QQKELCGSNK LVAPLGPTVA NPELFRKFGR
61GPEPWLGSVQ GQRSLLEHHP GKKQMGYMGE MEVQGPTRES GQSLPPQKKA YLSHLSTGSG
121HIEGDWAGRN RKLLKPRSIQ KSWFVQFPWL IMNEEQTALF CSACREYPSI RDKRSRLIEG
181YTGPFKVETL KYHAKSKAHM FCVNALAARD PIWAARFRSI RDPPGDVLAS PEPLFTADCP
241IFYPPGPLGG FDSMAELLPS SRAELEDPGG DGAIPAMYLD CISDLRQKEI TDGIHSSSDI
301NILYNDAVES CIQDPSAEGL SEEVPVVFEE LPVVFEDVAV YFTREEWGML DKRQKELYRD
361VMRMNYELLA SLGPAAAKPD LISKLERRAA PWIKDPNGPK WGKGRPPGNK KMVAVREADT
421QASAADSALL PGSPVEARAS CCSSSICEEG DGPRRIKRTY RPRSIQRSWF GQFPWLVIDP
481KETKLFCSAC IERPNLHDKS SRLVRGYTGP FKVETLKYHE VSKAHRLCVN TVEIKEDTPH
541TALVPEISSD LMANMEHFFN AAYSIAYHSR PLNDFEKILQ LLQSTGTVIL GKYRNRTACT
601QFIKYISETL KREILEDVRN SPCVSVLLDS STDASEQACV GIYIRYFKQM EVKESYITLA
661PLYSETADGY FETIVSALDE LDIPFRKPGW VVGLGTDGSA MLSCRGGLVE KFQEVIPQLL
721PVHCVAHRLH LAVVDACGSI DLVKKCDRHI RTVFKFYQSS NKRLNELQEG AAPLEQEIIR
781LKDLNAVRWV ASRRRTLHAL LVSWPALARH LQRVAEAGGQ IGHRAKGMLK LMRGFHFVKF
841CHFLLDFLSI YRPLSEVCQK EIVLITEVNA TLGRAYVALE SLRHQAGPKE EEFNASFKDG
901RLHGICLDKL EVAEQRFQAD RERTVLTGIE YLQQRFDADR PPQLKNMEVF DTMAWPSGIE
961LASFGNDDIL NLARYFECSL PTGYSEEALL EEWLGLKTIA QHLPFSMLCK NALAQHCRFP
1021LLSKLMAVVV CVPISTSCCE RGFKAMNRIR TDERTKLSNE VLNMLMMTAV NGVAVTEYDP
1081QPAIQHWYLT SSGRRFSHVY TCAQVPARSP ASARLRKEEM GALYVEEPRT QKPPILPSRE
1141AAEVLKDCIM EPPERLLYPH TSQEAPGMS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1169 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.