Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF851 / ZBTB44 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101166-100
Catalog Number: LS-G101166
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BTBD15(ZBTB44) (NM_014155), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BTB (POZ) domain containing 15 | BTBD15 | HSPC063 | ZBTB44 | Zinc finger protein 851 | ZNF851
- Origin Species
- Human
- NCBI GenBank
- NM_014155
- SwissProt
- Q8NCP5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGVKTFTHSS SSHSQEMLGK LNMLRNDGHF CDITIRVQDK IFRAHKVVLA ACSDFFRTKL61VGQAEDENKN VLDLHHVTVT GFIPLLEYAY TATLSINTEN IIDVLAAASY MQMFSVASTC121SEFMKSSILW NTPNSQPEKG LDAGQENNSN CNFTSRDGSI SPVSSECSVV ERTIPVCRES181RRKRKSYIVM SPESPVKCGT QTSSPQVLNS SASYSENRNQ PVDSSLAFPW TFPFGIDRRI241QPEKVKQAEN TRTLELPGPS ETGRRMADYV TCESTKTTLP LGTEEDVRVK VERLSDEEVH301EEVSQPVSAS QSSLSDQQTV PGSEQVQEDL LISPQSSSIG SVDEGVSEGL PTLQSTSSTN361APPDDDDRLE NVQYPYQLYI APSTSSTERP SPNGPDRPFQ CPTCGVRFTR IQNLKQHMLI421HSGIKPFQCD RCGKKFTRAY SLKMHRLKHE VISTRTRPLEQKLISEEDLAANDILDYKDDDDKV
453 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.