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ZNF837 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF837 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF837 antibodies →
$504.00each
Catalog Number: LS-G127334-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF837 (NM_138466), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 837 | ZNF837
Origin Species
Human
SwissProt
Q96EG3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAPAQKAGQ GGLPKADAQG ASGAREKRPE EPRPLEEDRA GSRPTQKGDL RGAAGGRTTP
61PGGGSRGCSL GVSPGPGTRH SAGTRPLVRE PCGPTSSQNP ELVIPEGLQA REGPCRSPAR
121GGDCSRNSCL AWHRGAPAGE TPPVCDPCPE RIQNHPRTQL CEVHTDCWPC QPGTGAPTCP
181RTPKPTSRGR NPLVEQPRAC ACGEAFAWRA LRIPQERLQA TEEPRPCARC GKRFRPNQQQ
241QAGKSPPVCP ECGQTSRPRP IVPDPPAQRL YACDECGKAF TRTSSLLQHQ RIHTGERPYE
301CAECGKAFVR CSGLYRHQKT HSAERHRRGP VLARRAFRLG CPPCGDYSER SPRRGSGAGE
361KPYECADCAK AFGLFSHLVE HRRVHTGEKP YACPECGKAF NQRSNLSRHQ RTHSSAKPYA
421CPLCEKAFKG RSGLVQHQRA HTGERPYGCS ECGKTFRGCS ELRQHERLHS GEKPYICRDC
481GKAFVRNCSL VRHLRTHTGE RPYACGDCGR AFSQRSNLNE HRKRHGGRAA P
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.