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ZNF829 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF829 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129944-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF829 (NM_001171979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 829 | ZNF829
Origin Species
Human
SwissProt
Q3KNS6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTGKGLGEDT EDRWGAGALG PCGRSGLKGG GCVWEAEAGG SRGQEIETIL ANMETSLRSG
61QIPTLDSSEH NLSPEPLELD RMPHSPLISI PHVWCHPEEE ERMHDELLQA VSKGPVMFRD
121VSIDFSQEEW ECLDADQMNL YKEVMLENFS NLVSVGLSNS KPAVISLLEQ GKEPWMVDRE
181LTRGLCSDLE SMCETKILSL KKRHFSQVII TREDMSTFIQ PTFLIPPQKT MSEEKPWECK
241ICGKTFNQNS QFIQHQRIHF GEKHYESKEY GKSFSRGSLV TRHQRIHTGK KPYECKECGK
301AFSCSSYFSQ HQRIHTGEKP YECKECGKAF KYCSNLNDHQ RIHTGEKPYE CKVCGKAFTK
361SSQLFLHLRI HTGEKPYECK ECGKAFTQHS RLIQHQRMHT GEKPYECKQC GKAFNSASTL
421TNHHRIHAGE KLYECEECRK AFIQSSELIQ HQRIHTDEKP YECNECGKAF NKGSNLTRHQ
481RIHTGEKPYD CKECGKAFGS RSDLIRHEGI HTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.