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ZNF827 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF827 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF827 antibodies →
$725.00each
Catalog Number: LS-G104598-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF827 (NM_178835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF827 | Zinc finger protein 827
Origin Species
Human
SwissProt
Q17R98

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
118.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRRKQEQPK RLPSHVSRQE EAEGELSEGE HWYGNSSETP SEASYGEVQE NYKLSLEDRI
61QEQSTSPDTS LGSTTPSSHT LELVALDSEV LRDSLQCQDH LSPGVSSLCD DDPGSNKPLS
121SNLRRLLEAG SLKLDAAATA NGRVESPVNV GSNLSFSPPS HHAQQLSVLA RKLAEKQEQN
181DQYTPSNRFI WNQGKWLPNS TTTCSLSPDS AILKLKAAAN AVLQDKSLTR TEETMRFESF
241SSPFSSQSAS STLAALSKKV SERSLTPGQE HPPPASSFLS LASMTSSAAL LKEVAARAAG
301SLLAEKSSLL PEDPLPPPPS EKKPEKVTPP PPPPPPPPPP PPPQSLELLL LPVPKGRVSK
361PSNSASEEES GKPFQCPICG LVIKRKSYWK RHMVIHTGLK SHQCPLCPFR CARKDNLKSH
421MKVHQHQDRG ETFQCQLCPF TSSRHFSLKL HMRCHQHFLR TEAKVKEEIP DPDVKGSPHL
481SDSACLGQQR EGGGTELVGT MMTSNTPERT SQGGAGVSPL LVKEEPKEDN GLPTSFTLNA
541ADRPANHTKL KDPSEYVANS ASALFSQDIS VKMASDFLMK LSAANQKEPM NLNFKVKEEP
601KEGESLSTTL PRSSYVFSPE SEVSAPGVSE DALKPQEGKG SVLRRDVSVK AASELLMKLS
661AESYKETQMV KIKEEPMEVD IQDSHVSISP SRNVGYSTLI GREKTEPLQK MPEGRVPPER
721NLFSQDISVK MASELLFQLS EKVSKEHNHT KENTIRTTTS PFFSEDTFRQ SPFTSNSKEL
781LPSDSVLHGR ISAPETEKIV LEAGNGLPSW KFNDQLFPCD VCGKVFGRQQ TLSRHLSLHT
841EERKYKCHLC PYAAKCRANL NQHLTVHSVK LVSTDTEDIV SAVTSEGSDG KKHPYYYSCH
901VCGFETELNV QFVSHMSLHV DKEQWMFSIC CTACDFVTME EAEIKTHIGT KHTGEDRKTP
961SESNSPSSSS LSALSDSANS KDDSDGSQKN KGGNNLLVIS VMPGSQPSLN SEEKPEKGFE
1021CVFCNFVCKT KNMFERHLQI HLITRMFECD VCHKFMKTPE QLLEHKKCHT VPTGGLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1077 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.