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ZNF823 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF823 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119651-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSZFP36(ZNF823) (NM_001080493), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSZFP36 | ZNF823 | Zinc finger protein ZFP-36 | Zinc finger protein 823
Origin Species
Human
NCBI GenBank
NM_001080493
SwissProt
P16415

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSVAFEDVA VNFTQEEWAL LGPSQKSLYR NVMQETIRNL DCIEMKWEDQ NIGDQCQNAK
61RNLRSHTCEI KDDSQCGETF GQIPDSIVNK NTPRVNPCDS GECGEVVLGH SSLNCNIRVD
121TGHKSCEHQE YGEKPYTHKQ RGKAISHQHS FQTHERPPTG KKPFDCKECA KTFSSLGNLR
181RHMAAHHGDG PYKCKLCGKA FVWPSLFHLH ERTHTGEKPY ECKQCSKAFP FYSSYLRHER
241IHTGEKAYEC KQCSKAFPDY STYLRHERTH TGEKPYKCTQ CGKAFSCYYY TRLHERTHTG
301EQPYACKQCG KTFYHHTSFR RHMIRHTGDG PHKCKICGKG FDCPSSVRNH ETTHTGEKPY
361ECKQCGKVLS HSSSFRSHMI THTGDGPQKC KICGKAFGCP SLFQRHERTH TGEKPYQCKQ
421CGKAFSLAGS LRRHEATHTG VKPYKCQCGK AFSDLSSFQN HETTHTGEKP YECKECGKAF
481SCFKYLSQHK RTHTVEKPYE CKTCRKAFSH FSNLKVHERI HSGEKPYECK ECGKAFSWLT
541CLLRHERIHT GEKPYECLQC GKAFTRSRFL RGHEKTHTGE KLYECKECGK ALSSLRSLHR
601HKRTHWKDTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.