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ZNF8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110781-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF8 (NM_021089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HF.18 | Zinc finger protein 8 | Zinc finger protein 272 | ZNF8 | Zfp128 | Zinc finger protein HF.18
Origin Species
Human
NCBI GenBank
M29581
SwissProt
P17098

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPEDEGVAG VMSVGPPAAR LQEPVTFRDV AVDFTQEEWG QLDPTQRILY RDVMLETFGH
61LLSIGPELPK PEVISQLEQG TELWVAERGT TQGCHPAWEP RSESQASRKE EGLPEEEPSH
121VTGREGFPTD APYPTTLGKD RECQSQSLAL KEQNNLKQLE FGLKEAPVQD QGYKTLRLRE
181NCVLSSSPNP FPEISRGEYL YTYDSQITDS EHNSSLVSQQ TGSPGKQPGE NSDCHRDSSQ
241AIPITELTKS QVQDKPYKCT DCGKSFNHNA HLTVHKRIHT GERPYMCKEC GKAFSQNSSL
301VQHERIHTGD KPYKCAECGK SFCHSTHLTV HRRIHTGEKP YECQDCGRAF NQNSSLGRHK
361RTHTGEKPYT CSVCGKSFSR TTCLFLHLRT HTEERPYECN HCGKGFRHSS SLAQHQRKHA
421GEKPFECRQR LIFEQTPALT KHEWTEALGC DPPLSQDERT HRSDRPFKCN QCGKCFIQSS
481HLIRHQITHT REEQPHGRSR RREQSSSRNS HLVQHQHPNS RKSSAGGAKA GQPESRALAL
541FDIQKIMQEK NPVHVIGVEE PSVGASMLFD IREST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

575 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.