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ZNF784 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF784 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103095-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF784 (NM_203374), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 784 | ZNF784
Origin Species
Human
SwissProt
Q8NCA9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
34.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAARPEAQS RSSPTPESRS QEPLDLVLVP DDCRPGTPPS DLIEIQVVKV TDTTLVPEPP
61EPGSFHCALC PAAFRLVSEL LFHEHGHLAG AEGGGQGGDP SRCHVCGHSC PGPASLRAHY
121SLHTGERPYR CALCPRAFKA LAPLLRHQHR HGVEPGTSRR PPDTAAVAEQ RPGVAPERAE
181VVMAAAAAGA AVGKPFACRF CAKPFRRSSD MRDHERVHTG ERPYHCGICG KGFTQSSVLS
241GHARIHTGER PFRCTLCDRT FNNSSNFRKH QRTHFHGPGP GLGDSGGQLG SSAAEGSGSG
301CGVGDPAEEG RGETAKVKVE ADQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

323 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.