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ZNF783 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF783 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G131027-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF783 (NM_001195220), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Protein ZNF783 | Zinc finger family member 783 | ZNF783
Origin Species
Human
SwissProt
Q6ZMS7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAAPARDP ETDKHTEDQS PSTPLPQPAA EKNSYLYSTE ITLWTVVAAI QALEKKVDSC
61LTRLLTLEGR TGTAEKKLAD CEKTAVEFGN QLEGKWAVLG TLLQEYGLLQ RRLENVENLL
121RNRNFWILRL PPGSKGEAPK VPVTFDDVAV YFSELEWGKL EDWQKELYKH VMRGNYETLV
181SLDYAISKPD ILTRIERGEE PCLDRWGQEK GNEVEVGRPR MMGTGLPPYP EHLTSPLSPA
241QEELKEGQAP KQQQDSEARV APAGPEAGGG VAIKTEAQSE DEMTPERLFL GVSRGQTECR
301IPRGPRNRPG GPSRHQAQGM PRVRAGEPRP PGASGETPRV LSRRRQRAFP CPDCGQSFRL
361KINLTIHQRT HVEEGRQEAP GRSPTSCGDS QAMLEPGEVV VPGPVIRWLP EEPEGRRSVA
421GGRALVGRRP AASKMYHCSE CLRFFQQRKS LLLHQRLHTG NGQGWPACPY CGKAFRRPSD
481LFRHQRIHTG ERPYQCPQCG RTFNRNHHLA VHMQTHARGQ VGPHFPAAPA RHGSLPLPWP
541SRKEEG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

546 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -80°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.