LSBio
ZNF774 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF774 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF774 antibodies →
$504.00each
Catalog Number: LS-G122565-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF774 (NM_001004309), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q6NX45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWLGTSGKSG LPGHCLENPL QECHPAQLEE WALKGISRPS VISQPEQKEE PWVLPLQNFE
61ARKIPRESHT DCEHQVAKLN QDSSETAEQC GTSSERTNKD LSHTLSWGGN WEQGLELEGQ
121HGTLPGEGQL ESFSQERDLN KLLDGYVGEK PMCAECGKSF NQSSYLIRHL RTHTGERPYT
181CIECGKGFKQ SSDLVTHRRT HTGEKPYQCK GCEKKFSDSS TLIKHQRTHT GERPYECPEC
241GKTFGRKPHL IMHQRTHTGE KPYACLECHK SFSRSSNFIT HQRTHTGVKP YRCNDCGESF
301SQSSDLIKHQ RTHTGERPFK CPECGKGFRD SSHFVAHMST HSGERPFSCP DCHKSFSQSS
361HLVTHQRTHT GERPFKCENC GKGFADSSAL IKHQRIHTGE RPYKCGECGK SFNQSSHFIT
421HQRIHLGDRP YRCPECGKTF NQRSHFLTHQ RTHTGEKPFH CSKCNKSFRQ KAHLLCHQNT
481HLI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

483 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.