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ZNF770 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF770 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114147-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF770 (NM_014106), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PRO1914 | ZNF770 | Zinc finger protein 770
Origin Species
Human
NCBI GenBank
NM_014106
SwissProt
Q6IQ21

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMAENNLKML KIQQCVVANK LPRNRPYVCN ICFKHFETPS KLARHYLIHT GQKPFECDVC
61HKTFRQLVHL ERHQLTHSLP FKCSICQRHF KNLKTFVKHQ QLHNETYQNN VKQVRRLLEA
121KQEKSMYGVY NTFTTEERWA LHPCSKSDPM YSMKRRKNIH ACTICGKMFP SQSKLDRHVL
181IHTGQRPFKC VLCTKSFRQS THLKIHQLTH SEERPFQCCF CQKGFKIQSK LLKHKQIHTR
241NKAFRALLLK KRRTESRPLP NKLNANQGGF ENGEIGESEE NNPLDVHSIY IVPFQCPKCE
301KCFESEQILN EHSCFAARSG KIPSRFKRSY NYKTIVKKIL AKLKRARSKK LDNFQSEKKV
361FKKSFLRNCD LISGEQSSEQ TQRTFVGSLG KHGTYKTIGN RKKKTLTLPF SWQNMGKNLK
421GILTTENILS IDNSVNKKDL SICGSSGEEF FNNCEVLQCG FSVPRENIRT RHKICPCDKC
481EKVFPSISKL KRHYLIHTGQ RPFGCNICGK SFRQSAHLKR HEQTHNEKSP YASLCQVEFG
541NFNNLSNHSG NNVNYNASQQ CQAPGVQKYE VSESDQMSGV KAESQDFIPG STGQPCLPNV
601LLESEQSNPF CSYSEHQEKN DVFLYRCSVC AKSFRSPSKL ERHYLIHAGQ KPFECSVCGK
661TFRQAPHWKR HQLTHFKERP QGKVVALDSV M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

691 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.