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ZNF77 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF77 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110706-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF77 (NM_021217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF77 | ZNFpT1 | Zinc finger protein 77 | Zinc finger protein 77 (pT1) | PT1
Origin Species
Human
NCBI GenBank
AL365370
SwissProt
Q15935

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDCVIFEEVA VNFTPEEWAL LDHAQRSLYR DVMLETCRNL ASLDCYIYVR TSGSSSQRDV
61FGNGISNDEE IVKFTGSDSW SIFGENWRFD NTGDQHQIPQ RHLRSQLGRL CESNEGHQCG
121ETLSQTANLL VHKSYPTEAK PSECTKCGKA FENRQRSHTG QRPCKECGQA CSCLSCQSPP
181MKTQTVEKPC NCQDSRTASV TYVKSLSSKK SYECQKCGKA FICPSSFRGH VNSHHGQKTH
241ACKVCGKTFM YYSYLTRHVR THTGEKPYEC KECGKAFSCP SYFREHVRTH TGEKPYECKH
301CGKSFSCYSS FRDHVRTHTG EKPCQCKHCG KAFTCYSSLR EHGRTHSGEK PYECKECGKA
361FRYPSSLRAH MRMHTGEKPY VCKQCGKAFG CPTYFRRHVK THSGVKPYQC KECGKAYSFS
421SSLRIHVRTH TGEKPFECKH CGKAFSCHSS LREHVRTHSG EKPYECNQCG KAFSHAQYFQ
481KHVRSHSGVK PYECTECGKA YSCSSSLRVH VRTHTGERPY ECKQCGKTFR YLASLQAHVR
541THAGA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.