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ZNF76 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF76 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF76 antibodies →
$504.00each
Catalog Number: LS-G117340-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF76 (NM_003427), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D6S229E | Zinc finger protein 76 | ZNF523 | Zfp523 | Zinc finger protein 523 | ZNF76
Origin Species
Human
NCBI GenBank
NM_003427
SwissProt
P36508

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESLGLHTVT LSDGTTAYVQ QAVKGEKLLE GQVIQLEDGT TAYIHQVTVQ KEALSFEDGQ
61PVQLEDGSMA YIHRTPREGY DPSTLEAVQL EDGSTAYIHH PVAVPSESTI LAVQTEVGLE
121DLAAEDDEGF SADAVVALEQ YASKVLHDSQ IPRNGKGQQV GDRAFRCGYK GCGRLYTTAH
181HLKVHERAHT GDRPYRCDFP SCGKAFATGY GLKSHVRTHT GEKPYKCPEE LCSKAFKTSG
241DLQKHVRTHT GERPFQCPFE GCGRSFTTSN IRKVHVRTHT GERPYTCPEP HCGRGFTSAT
301NYKNHVRIHT GEKPYVCTVP GCGKRFTEYS SLYKHHVVHT HCKPYTCSTC GKTYRQTSTL
361AMHKRSAHGE LEATEESEQA LYEQQQLEAA SAAEESPPPK RPRIAYLSEV KEERDDIPAQ
421VAMVTEEDGA PQVALITQDG AQQVSLSPED LQALGSAISM VTQHGSTTLT IPSPDADLAT
481SGTHTVTMVS ADGTQTQPVT IITSGAVVAE DSSVASLRHQ QVALLATANG THIAVQLEEQ
541QTLEEAINVA TAAMQQGAVT LETTVSESGC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.